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The characterisation of sperm and seminal plasma glycoproteins: Their role within the tetraspanin network and male fertility
Páleníková, Veronika
Mgr. Veronika Páleníková only to describe the localization and heterodimerization of α3, α6, αV, β1, and β4 integrins in mentioned integrins. Importantly, we detected the presence of integrin β4 subunit in mouse sperm, and characterised that it forms functional heterodimer with α6 subunit. In tion, we described α6β1 and α3β1integrin pairs, which contribute to the integrity and complexity of sperm membranes. Moreover, we found that α6 integrin subunit interacts with proposed CD9 and CD81 as tetraspanin partners for αV integrin in tetraspanin web. Our goal
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The characterisation of sperm and seminal plasma glycoproteins: Their role within the tetraspanin network and male fertility
Páleníková, Veronika ; Postlerová, Pavla (advisor) ; Ryšlavá, Helena (referee) ; Pěknicová, Jana (referee)
Mgr. Veronika Páleníková only to describe the localization and heterodimerization of α3, α6, αV, β1, and β4 integrins in mentioned integrins. Importantly, we detected the presence of integrin β4 subunit in mouse sperm, and characterised that it forms functional heterodimer with α6 subunit. In tion, we described α6β1 and α3β1integrin pairs, which contribute to the integrity and complexity of sperm membranes. Moreover, we found that α6 integrin subunit interacts with proposed CD9 and CD81 as tetraspanin partners for αV integrin in tetraspanin web. Our goal
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The role of integrins in gametes prior to fertilization and during their interaction
Foldynová, Veronika ; Komrsková, Kateřina (advisor) ; Tlapáková, Tereza (referee)
Integrins are heterodimeric transmembrane glycoproteins that represent a large group of cell adhesion receptors involved in cell-cell, cell-extracellular matrix and cell-pathogen interacti- ons. Up to now, 24 different integrin heterodimers have been detected in mammals. They are involved in a wide range of processes such as immune response, lymphocyte homing, platelet aggregation, also in wound healing, cell differentiation, migration, proliferation and even in cell survival. Integrins have also been detected on germ cells and are now known to play an important role in reproductive processes such as fertilization, embryo implan- tation, and embryonic development. The main aim of this thesis is to introduce integrins from the perspective of reproduction, integrin physiology, occurrence and localization of individual subunits in male and female germ cells. A significant part of the work is devoted to a discussion of the role of integrins in gametes, both during maturation processes (egg maturation in the ovary, capacitation and the acrosomal reaction of sperm), in sperm mi- gration, oviductal reservoir formation and their direct and indirect involvement in adhesion and fusion of the gametes during fertilization. Keywords: integrins, sperm, egg, fusion, fertilization
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The role of integrins in gametes prior to fertilization and during their interaction
Foldynová, Veronika ; Komrsková, Kateřina (advisor) ; Tlapáková, Tereza (referee)
Integrins are heterodimeric transmembrane glycoproteins that represent a large group of cell adhesion receptors involved in cell-cell, cell-extracellular matrix and cell-pathogen interacti- ons. Up to now, 24 different integrin heterodimers have been detected in mammals. They are involved in a wide range of processes such as immune response, lymphocyte homing, platelet aggregation, also in wound healing, cell differentiation, migration, proliferation and even in cell survival. Integrins have also been detected on germ cells and are now known to play an important role in reproductive processes such as fertilization, embryo implan- tation, and embryonic development. The main aim of this thesis is to introduce integrins from the perspective of reproduction, integrin physiology, occurrence and localization of individual subunits in male and female germ cells. A significant part of the work is devoted to a discussion of the role of integrins in gametes, both during maturation processes (egg maturation in the ovary, capacitation and the acrosomal reaction of sperm), in sperm mi- gration, oviductal reservoir formation and their direct and indirect involvement in adhesion and fusion of the gametes during fertilization. Keywords: integrins, sperm, egg, fusion, fertilization
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The role of p130CAS in integrin signaling
Janoštiak, Radoslav ; Brábek, Jan (advisor) ; Brdička, Tomáš (referee) ; Kořínek, Vladimír (referee)
Focal adhesions are important subcellular structures that are composed of many signaling and scaffolding proteins. They serve not only for anchoring the cell to the substratum but they are also important signaling centers that regulate various cellular behavior such as migration, invasiveness, proliferation and survival. Focal adhesion signaling needs to be strictly regulated because alteration in activity or expression of many focal adhesion proteins leads to tumorogenesis and metastasis formation. One of the most important scaffolding protein associated with focal adhesion is p130Cas. The importance of p130Cas in regulation of cell migration and invasiveness has been well established. P130Cas also plays important role in regulation of cell survival and proliferation. Moreover, high protein levels of human ortholog of p130Cas - BCAR1, has been linked to more aggressive breast tumors and poor prognosis. During my doctoral studies, I focused on the role of p130Cas in integrin signaling. At the beginning we characterized the role of tyrosine 12 phosphorylation within its SH3 domain. We confirmed that this phosphorylation is increased in Src527F transformed mouse embryonic fibroblasts compared to non-transformed counterparts and also in some human cancer cell lines. We showed that this phosphorylation...
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